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KMID : 0545119990090030358
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Journal of Microbiology and Biotechnology
1999 Volume.9 No. 3 p.358 ~ p.360
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Improved Menthol Production Using Suspension Cultures of Mentha piperita with Pectinase Elicitation
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Chang, Jun Hyong
Shin, Joong Han/Chung, In Sik/Lee, Hyong Joo
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Abstract
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The effect of pectinase on menthol production by Mentha piperita in shake flasks was investigated. The optimum concentration of pectinase and period of elicitation for menthol production were 15U/l and 9 days, respectively. Pectinase elicitation at 15U/l for 9 days using a Lin-Staba medium with 2,4-dichlorophenoxyacetic acid (2,4-D) enhanced menthol production 37-fold (211.5㎎ menthol/l) with the specific menthol concentration (menthol concentration per unit weight of cells) of 27.5㎎/g dry cell weight (DCW). Our results also indicate that pectinase elicitation may activate the conversion of pulegone to menthol.
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